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pgex 6p3  (Danaher Inc)


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    Structured Review

    Danaher Inc pgex 6p3
    Pgex 6p3, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 94/100, based on 680 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pgex+6p3/pGEX-6P-3/pmc01405818-38-14-16
    Average 94 stars, based on 680 article reviews
    pgex 6p3 - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Recombinant:

    Article Title: PTPL1/FAP-1 Negatively Regulates TRIP6 Function in Lysophosphatidic Acid-induced Cell Migration
    Article Snippet: .. To express the recombinant PTPL1 catalytic domain (designated PTPL1-CD), a cDNA fragment encoding amino acids 2087–2485 of PTPL1 was amplified by PCR from pCMV5-HA-PTPL1 and inserted into pGEX-6P3 (Amersham Biosciences). ..

    Amplification:

    Article Title: PTPL1/FAP-1 Negatively Regulates TRIP6 Function in Lysophosphatidic Acid-induced Cell Migration
    Article Snippet: .. To express the recombinant PTPL1 catalytic domain (designated PTPL1-CD), a cDNA fragment encoding amino acids 2087–2485 of PTPL1 was amplified by PCR from pCMV5-HA-PTPL1 and inserted into pGEX-6P3 (Amersham Biosciences). ..

    Article Title: TRIP6 antagonizes the recruitment of A20 and CYLD to TRAF6 to promote the LPA2 receptor-mediated TRAF6 activation
    Article Snippet: .. The cDNA sequences of TRAF6 or its truncation mutant containing either the RING domain (residues 1–149), zinc-finger domains (residues 119–238) or carboxyl-terminal domain (residues 239–530) were amplified by polymerase chain reaction using pCMV5-FLAG-TRAF6 (Addgene, Cambridge, MA, USA) [ ] as the template, and were inserted in-frame into pEGFP-C1 (Clontech, Mountain View, CA, USA) or pGEX-6P3 (Amersham Biosciences, Piscataway, NJ, USA). .. The FLAG-E52A-TRIP6 expression vector was constructed by QuikChange site-directed mutagenesis (Agilent, Santa Clara, CA, USA) using pCMV-FLAG-TRIP6 as the template.

    Article Title: TRIP6 Regulates p27 KIP1 To Promote Tumorigenesis
    Article Snippet: .. The cDNA sequences encoding p27 KIP1 , TRIP6, lipoma preferred partner (LPP), zyxin, AKT1, or a truncation mutant of p27 KIP1 or TRIP6 were amplified by PCR and inserted in frame into pCMV-Tag2A (Stratagene), pcDNA3-HA, pmCherry-C1, pEGFP-C1 (Clontech), or pGEX-6P3 (Amersham Biosciences). ..

    Article Title: Model of OSBP-Mediated Cholesterol Supply to Aichi Virus RNA Replication Sites Involving Protein-Protein Interactions among Viral Proteins, ACBD3, OSBP, VAP-A/B, and SAC1
    Article Snippet: 550 Guinea pig antibodies that recognize either VAPA or VAPB were prepared (Medical & 551 Biological Laboratories co., LTD. [MBL], Nagoya, Japan) by using the variable central 552 domain of VAPA and VAPB as antigens according to Teuling et al. (61). .. The VAPA 553 nucleotide sequence encoding aa 139-232 and the VAPB nucleotide sequence encoding 554 aa 132-225 were amplified from pACT-VAPA using the primers, Bam-VAPA-415Fw 555 (5’-AAGGATCCTTGAATGATATGGAACCTAGC-3’) and Sal-VAPA-696Rv 556 (5’-AAAGTCGACTTAAAGAAGTGAAGGAAGAGG-3’) and from pACT-VAPB 557 using the primers, Bam-VAPB-394Fw 558 (5’-AAGGATCCCCACATGATGTAGAAATAAATA-3’) and Sal-VAPB-675Rv 559 (5’-AAAGTCGACTTACAAGAGCCGGGTGCTAAG-3’), respectively, and cloned 560 into pGEX-6P3 (Amersham Pharmacia Biotech). .. The GST-fusion proteins were 561 expressed in Escherichia coli cells as described in the manufacturer’s protocol, and 562 on January 26, 2018 by F U D A N U N IV E R S IT Y http://jvi.asm .org/ D ow nloaded from purified as described previously (23), and antibodies against these proteins were raised 563 in guinea pigs (MBL, Nagoya, Japan).

    Polymerase Chain Reaction:

    Article Title: PTPL1/FAP-1 Negatively Regulates TRIP6 Function in Lysophosphatidic Acid-induced Cell Migration
    Article Snippet: .. To express the recombinant PTPL1 catalytic domain (designated PTPL1-CD), a cDNA fragment encoding amino acids 2087–2485 of PTPL1 was amplified by PCR from pCMV5-HA-PTPL1 and inserted into pGEX-6P3 (Amersham Biosciences). ..

    Article Title: TRIP6 antagonizes the recruitment of A20 and CYLD to TRAF6 to promote the LPA2 receptor-mediated TRAF6 activation
    Article Snippet: .. The cDNA sequences of TRAF6 or its truncation mutant containing either the RING domain (residues 1–149), zinc-finger domains (residues 119–238) or carboxyl-terminal domain (residues 239–530) were amplified by polymerase chain reaction using pCMV5-FLAG-TRAF6 (Addgene, Cambridge, MA, USA) [ ] as the template, and were inserted in-frame into pEGFP-C1 (Clontech, Mountain View, CA, USA) or pGEX-6P3 (Amersham Biosciences, Piscataway, NJ, USA). .. The FLAG-E52A-TRIP6 expression vector was constructed by QuikChange site-directed mutagenesis (Agilent, Santa Clara, CA, USA) using pCMV-FLAG-TRIP6 as the template.

    Article Title: TRIP6 Regulates p27 KIP1 To Promote Tumorigenesis
    Article Snippet: .. The cDNA sequences encoding p27 KIP1 , TRIP6, lipoma preferred partner (LPP), zyxin, AKT1, or a truncation mutant of p27 KIP1 or TRIP6 were amplified by PCR and inserted in frame into pCMV-Tag2A (Stratagene), pcDNA3-HA, pmCherry-C1, pEGFP-C1 (Clontech), or pGEX-6P3 (Amersham Biosciences). ..

    Mutagenesis:

    Article Title: TRIP6 antagonizes the recruitment of A20 and CYLD to TRAF6 to promote the LPA2 receptor-mediated TRAF6 activation
    Article Snippet: .. The cDNA sequences of TRAF6 or its truncation mutant containing either the RING domain (residues 1–149), zinc-finger domains (residues 119–238) or carboxyl-terminal domain (residues 239–530) were amplified by polymerase chain reaction using pCMV5-FLAG-TRAF6 (Addgene, Cambridge, MA, USA) [ ] as the template, and were inserted in-frame into pEGFP-C1 (Clontech, Mountain View, CA, USA) or pGEX-6P3 (Amersham Biosciences, Piscataway, NJ, USA). .. The FLAG-E52A-TRIP6 expression vector was constructed by QuikChange site-directed mutagenesis (Agilent, Santa Clara, CA, USA) using pCMV-FLAG-TRIP6 as the template.

    Article Title: TRIP6 Regulates p27 KIP1 To Promote Tumorigenesis
    Article Snippet: .. The cDNA sequences encoding p27 KIP1 , TRIP6, lipoma preferred partner (LPP), zyxin, AKT1, or a truncation mutant of p27 KIP1 or TRIP6 were amplified by PCR and inserted in frame into pCMV-Tag2A (Stratagene), pcDNA3-HA, pmCherry-C1, pEGFP-C1 (Clontech), or pGEX-6P3 (Amersham Biosciences). ..

    Sequencing:

    Article Title: Model of OSBP-Mediated Cholesterol Supply to Aichi Virus RNA Replication Sites Involving Protein-Protein Interactions among Viral Proteins, ACBD3, OSBP, VAP-A/B, and SAC1
    Article Snippet: 550 Guinea pig antibodies that recognize either VAPA or VAPB were prepared (Medical & 551 Biological Laboratories co., LTD. [MBL], Nagoya, Japan) by using the variable central 552 domain of VAPA and VAPB as antigens according to Teuling et al. (61). .. The VAPA 553 nucleotide sequence encoding aa 139-232 and the VAPB nucleotide sequence encoding 554 aa 132-225 were amplified from pACT-VAPA using the primers, Bam-VAPA-415Fw 555 (5’-AAGGATCCTTGAATGATATGGAACCTAGC-3’) and Sal-VAPA-696Rv 556 (5’-AAAGTCGACTTAAAGAAGTGAAGGAAGAGG-3’) and from pACT-VAPB 557 using the primers, Bam-VAPB-394Fw 558 (5’-AAGGATCCCCACATGATGTAGAAATAAATA-3’) and Sal-VAPB-675Rv 559 (5’-AAAGTCGACTTACAAGAGCCGGGTGCTAAG-3’), respectively, and cloned 560 into pGEX-6P3 (Amersham Pharmacia Biotech). .. The GST-fusion proteins were 561 expressed in Escherichia coli cells as described in the manufacturer’s protocol, and 562 on January 26, 2018 by F U D A N U N IV E R S IT Y http://jvi.asm .org/ D ow nloaded from purified as described previously (23), and antibodies against these proteins were raised 563 in guinea pigs (MBL, Nagoya, Japan).

    Clone Assay:

    Article Title: Model of OSBP-Mediated Cholesterol Supply to Aichi Virus RNA Replication Sites Involving Protein-Protein Interactions among Viral Proteins, ACBD3, OSBP, VAP-A/B, and SAC1
    Article Snippet: 550 Guinea pig antibodies that recognize either VAPA or VAPB were prepared (Medical & 551 Biological Laboratories co., LTD. [MBL], Nagoya, Japan) by using the variable central 552 domain of VAPA and VAPB as antigens according to Teuling et al. (61). .. The VAPA 553 nucleotide sequence encoding aa 139-232 and the VAPB nucleotide sequence encoding 554 aa 132-225 were amplified from pACT-VAPA using the primers, Bam-VAPA-415Fw 555 (5’-AAGGATCCTTGAATGATATGGAACCTAGC-3’) and Sal-VAPA-696Rv 556 (5’-AAAGTCGACTTAAAGAAGTGAAGGAAGAGG-3’) and from pACT-VAPB 557 using the primers, Bam-VAPB-394Fw 558 (5’-AAGGATCCCCACATGATGTAGAAATAAATA-3’) and Sal-VAPB-675Rv 559 (5’-AAAGTCGACTTACAAGAGCCGGGTGCTAAG-3’), respectively, and cloned 560 into pGEX-6P3 (Amersham Pharmacia Biotech). .. The GST-fusion proteins were 561 expressed in Escherichia coli cells as described in the manufacturer’s protocol, and 562 on January 26, 2018 by F U D A N U N IV E R S IT Y http://jvi.asm .org/ D ow nloaded from purified as described previously (23), and antibodies against these proteins were raised 563 in guinea pigs (MBL, Nagoya, Japan).

    Bacteria:

    Article Title: A tripartite DNA-binding element, comprised of the nuclear localization signal and two AT-hook motifs, mediates the association of LEDGF/p75 with chromatin in vivo
    Article Snippet: .. Deletion mutants were expressed in bacteria as fusions to glutathione S -transferase (GST) using pGEX-6P3-based vectors (Amersham Biosciences). ..



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